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non template controls  (ATCC)


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    ATCC non template controls
    Non Template Controls, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+virus/Synthetic+West+Nile+Virus+RNA/pm42087176-69-5-21
    Average 99 stars, based on 5 article reviews
    non template controls - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Virus:

    Article Title: Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences
    Article Snippet: .. Table 2 Respiratory syncytial virus reference controls used to standardise the RSVAB-WGS and RSVAB-GF sequencing methods Strain ATCC nomenclature Cq value Number of copies/mL Dilution of control virus 10-1 10-2 10-3 10-4 10-1 10-2 10-3 10-4 RSV A Long VR-26 15.12 18.88 22.09 25.57 2 × 104 2 × 103 2 × 102 2 × 101 RSV B CH-18537 VR-1580 16.21 19.5 22.8 26.2 2 × 104 2 × 103 2 × 102 NA ATCC: American type culture collection; Cq: quantification cycle; NA: not applicable. ..

    Sequencing:

    Article Title: Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences
    Article Snippet: .. Table 2 Respiratory syncytial virus reference controls used to standardise the RSVAB-WGS and RSVAB-GF sequencing methods Strain ATCC nomenclature Cq value Number of copies/mL Dilution of control virus 10-1 10-2 10-3 10-4 10-1 10-2 10-3 10-4 RSV A Long VR-26 15.12 18.88 22.09 25.57 2 × 104 2 × 103 2 × 102 2 × 101 RSV B CH-18537 VR-1580 16.21 19.5 22.8 26.2 2 × 104 2 × 103 2 × 102 NA ATCC: American type culture collection; Cq: quantification cycle; NA: not applicable. ..

    Control:

    Article Title: Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences
    Article Snippet: .. Table 2 Respiratory syncytial virus reference controls used to standardise the RSVAB-WGS and RSVAB-GF sequencing methods Strain ATCC nomenclature Cq value Number of copies/mL Dilution of control virus 10-1 10-2 10-3 10-4 10-1 10-2 10-3 10-4 RSV A Long VR-26 15.12 18.88 22.09 25.57 2 × 104 2 × 103 2 × 102 2 × 101 RSV B CH-18537 VR-1580 16.21 19.5 22.8 26.2 2 × 104 2 × 103 2 × 102 NA ATCC: American type culture collection; Cq: quantification cycle; NA: not applicable. ..



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    LUCas enables temporal barcoding assays for detecting multiple targets in a single reaction. ( A ) Schematic representation of the temporal barcoding assay, which enables differentiation between the trans -cleavage activity of one standard guide RNA (gRNA) and one photocleavable guide (pc-igRNA) in a single CRISPR reaction. ( B ) Demonstration of the LUCas assay for detecting two viruses (FluA and <t>SARS-CoV-2)</t> in a single reaction using a temporal-barcoding approach. The standard guide targets FluA (gRNA FluA), while the photocleavable guide is complementary to a segment of the SARS-CoV-2 virus genome. The curves show the mean of three replicates, and the shaded regions indicate the SEM. ( C ) The distinct temporal barcodes, based on measured rate changes (pre-stim and post-stim), allow distinguishing between the four possible outcomes in a single reaction. Error bars represent the mean ± SEM ( n = 3). ( D ) Measured cleaved reporter concentration over time of a reaction with a mixture of different target concentration ratios. Error bars represent the SEM ( n = 3). ( E ) Scatter plot of normalized slope difference \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $(R^{\prime }_{post-stim} - R^{\prime }_{pre-stim})/R^{\prime }_{pre-stim}$\end{document} , where \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $R^{\prime }$\end{document} is the reaction slope measured using a linear fit, and ratio of target concentrations shows a linear relationship, as expected. This relationship can be used to quantitatively estimate the target concentration ratios of a sample, given knowledge about individual guide-dependent activity. Data points show the mean ± SEM ( n = 3).
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    Image Search Results


    LUCas enables temporal barcoding assays for detecting multiple targets in a single reaction. ( A ) Schematic representation of the temporal barcoding assay, which enables differentiation between the trans -cleavage activity of one standard guide RNA (gRNA) and one photocleavable guide (pc-igRNA) in a single CRISPR reaction. ( B ) Demonstration of the LUCas assay for detecting two viruses (FluA and SARS-CoV-2) in a single reaction using a temporal-barcoding approach. The standard guide targets FluA (gRNA FluA), while the photocleavable guide is complementary to a segment of the SARS-CoV-2 virus genome. The curves show the mean of three replicates, and the shaded regions indicate the SEM. ( C ) The distinct temporal barcodes, based on measured rate changes (pre-stim and post-stim), allow distinguishing between the four possible outcomes in a single reaction. Error bars represent the mean ± SEM ( n = 3). ( D ) Measured cleaved reporter concentration over time of a reaction with a mixture of different target concentration ratios. Error bars represent the SEM ( n = 3). ( E ) Scatter plot of normalized slope difference \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $(R^{\prime }_{post-stim} - R^{\prime }_{pre-stim})/R^{\prime }_{pre-stim}$\end{document} , where \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $R^{\prime }$\end{document} is the reaction slope measured using a linear fit, and ratio of target concentrations shows a linear relationship, as expected. This relationship can be used to quantitatively estimate the target concentration ratios of a sample, given knowledge about individual guide-dependent activity. Data points show the mean ± SEM ( n = 3).

    Journal: Nucleic Acids Research

    Article Title: LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAs

    doi: 10.1093/nar/gkag848

    Figure Lengend Snippet: LUCas enables temporal barcoding assays for detecting multiple targets in a single reaction. ( A ) Schematic representation of the temporal barcoding assay, which enables differentiation between the trans -cleavage activity of one standard guide RNA (gRNA) and one photocleavable guide (pc-igRNA) in a single CRISPR reaction. ( B ) Demonstration of the LUCas assay for detecting two viruses (FluA and SARS-CoV-2) in a single reaction using a temporal-barcoding approach. The standard guide targets FluA (gRNA FluA), while the photocleavable guide is complementary to a segment of the SARS-CoV-2 virus genome. The curves show the mean of three replicates, and the shaded regions indicate the SEM. ( C ) The distinct temporal barcodes, based on measured rate changes (pre-stim and post-stim), allow distinguishing between the four possible outcomes in a single reaction. Error bars represent the mean ± SEM ( n = 3). ( D ) Measured cleaved reporter concentration over time of a reaction with a mixture of different target concentration ratios. Error bars represent the SEM ( n = 3). ( E ) Scatter plot of normalized slope difference \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $(R^{\prime }_{post-stim} - R^{\prime }_{pre-stim})/R^{\prime }_{pre-stim}$\end{document} , where \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $R^{\prime }$\end{document} is the reaction slope measured using a linear fit, and ratio of target concentrations shows a linear relationship, as expected. This relationship can be used to quantitatively estimate the target concentration ratios of a sample, given knowledge about individual guide-dependent activity. Data points show the mean ± SEM ( n = 3).

    Article Snippet: Finally, 14 mM of magnesium acetate, 2 μ l of synthetic SARS-CoV-2 RNA virus (Twist Bioscience, Cat#102019) and 25 nM of RNP were added, resulting in a 20 μ l final reaction volume.

    Techniques: Activity Assay, CRISPR, Virus, Concentration Assay